Docetaxel taxotere cas 114977285 abmole bioscience. Regional delivery of chimeric antigen receptorengineered t. Plates were read at 450 nm using the wallac victor3 1420 multilabel counter perkinelmer and wallac 1420 workstation software. The plate was analyzed using a victor 3 1420 multilabel plate reader with the wallac 1420 workstation software perkin elmer, waltham, ma with a 544. Surrogate indices of steatosis andor parameters of impaired fat metabolism aspartate aminotransferase ast, alanine transaminase alt, hepatic total lipids, hepatic triglycerides and hepatic total cholesterol were measured as previously described 34. Molecular sensors of estrogen receptor conformations and.
Atp binding and stabilization of the luminescent signal were performed according to manufacturers protocol promega following tumor cell lysis. Right click on the link to the file and choose save target as and save the zip file to your desktop. Operating wallac 1420 18 type the appropriate useridpassword combination to proceed. Clicking the change password opens the change password dialogue where you can define a new password. Samples were measured on wallac victor 1420 multilabel counter using wallac 1420 workstation software version 2 wallac oy, turku, finland. After a successful login wallac 1420 can be used according to the permissions granted to the logged on user. Dec 01, 2003 fluorescence experiments used a spex fluorolog ii model iiic cuvettebased fluorometer with data max 2. The wallac model 1409001 liquid scintillation counter and wallac model 1480011 gamma counter are used to detect radioactive contamination in samples collected, prepared and counted on these two devices. Preventive maintenance and repair on wallac liquid.
Nov 12, 20 absorbance, or overall plate functional activity, was measured at 590 nm every 12 h up to 120 h or until the average plate absorbance reached 0. The most popular version of this product among our users is 1. Perkin elmerwallac victor 2 multilabel counter iet. May 05, 2009 plates were read by using a victor 3 plate reader perkinelmer and the data analyzed by using wallac 1420 workstation software. Oct 15, 2016 no nets are visible in the control samples. Open wallac folder to see all available factory set protocols. Absorbance, timeresolved fluorescence trf, luminescence, fluorescence, fluorescence polarization. Mar 11, 2015 the plate was analyzed using a victor 3 1420 multilabel plate reader with the wallac 1420 workstation software perkin elmer, waltham, ma with a 544nm excitation filter and a 600nm emission filter. Wallac 1420 workstation perkinelmer life sciences software. Download wallac 1420 multilabel counter manual pdf user manual 1420 software version manual not relevant to the version of wallac 1420 you have. If you continue to use this site we will assume that you are happy with it. Operating wallac 1420 19 when the workstation is used without an instrument i. Fold induction was calculated as rlu in inoculated cells divided by rlu in noninoculated cells. Energetics and surface properties of pseudomonas putida.
Myd88 dependence of berylliuminduced dendritic cell. Acute respiratory distress syndrome neutrophils have a. Wallac 1420 workstation software, perkin elmer victor, tecan plate washers, as well as plate assay techniques. The luminometer expresses luminescence as counts per second cps. Plates were read by using a victor 3 plate reader perkinelmer and the data analyzed by using wallac 1420 workstation software. When wallac 1420 workstation is connected to an instrument running in normal mode, the demo mode indicator is blank. We own wallac 1420 multilabel counter user manual pdf, doc, epub, txt, djvu forms. The victor 2 can accommodate plates in formats between 6 and 864 wells read more. Molecular sensors of estrogen receptor conformations and dynamics. The wallac 1420 victor 2 is a multilabel, an extended version of the successful wallac victor multilabel reader, wallac 1420 manual wallac perkin elmer 1420 041 victor2 v plate reader perkin elmer wallac 1420 victor 2 multilabel counter. We report specific interactions between the transmembrane domains tmds of bax and the prosurvival bclxl bcell. Wallac 1420 multilabel counter the wallac 1420 multilabel counter is a complete platform for quantitative detection of light emitting, or light absorbing markers. In vivo tumor studies all animal experiments were performed under protocols approved by the city of hope institutional animal care and use committee.
The substrate solution was added, and the stop solution was added to each well after 25 minutes. Absorbance was read on a victor v multilabel plate reader perkinelmer, gaithersburg, md at a wavelength of a595 and data collected using wallac 1420 workstation software. Small molecule inhibitors of apaf1related caspase 39. Perkin elmerwallac victor 3v multilabel microplate reader for. Use the links below to quickly find your software solution download. Postoperative cognitive changes after total knee arthroplast. Refurbished perkin elmer wallac victor 2 multilabel counter. Perkin elmer wallac 1420 victor2 microplate reader gmi. Page 22 operating wallac 1420 you can choose the result range you want used to determine the colour code of the wells. Perkin elmer 1420 victor 3vincludes computer, software, looks greatavailable due to funding stopped at business. The wallac 1420 victor 2 is a multilabel, an extended version of the successful wallac victor multilabel reader, wallac 1420 manual wallac perkin elmer 1420041 victor2 v plate reader perkin elmer.
L were added to the required wells and the plates incubated for a further 4 h under the conditions. The recruitment of the predominantly cytosolic bcl2 protein bax bcl2 associated x, apoptosis regulator to the mitochondria is associated with mitochondrial outer membrane permeabilization and apoptosis. Windows workstation software provides a userfriendly interface for defining protocols and integrating with other automated systems, making the victor 2 as convenient as it is flexible. The most popular versions of this product among our users are. The photometer was equipped with two dispensers that were able to pump adjustable amounts of trisbuffer and luciferinluciferase solution into the wells of the microtiter plate, just. Measures fluorescence, luminescence, and absorbance. Depending on the exact model, it is suitable for flash or glow luminometry, fluorometry, highsensitivity timeresolved fluorometry.
All of these have a lock icon, so they cannot be edited. Data analysis was performed using the wallac 1420 workstation software wallac 1420 manager, version 2. Bax transmembrane domain interacts with prosurvival bcl2. Plates were read at 450 nm using a wallac victor3 1420 counter perkinelmer and the wallac 1420 workstation software.
Perkinelmer life and analytical sciences, wallac oy, p. D the kinetics of net formation was assessed over 4 hours by measuring total fluorescence using a victor 3 multilabel reader using wallac 1420 workstation v3. Fluorescence experiments used a spex fluorolog ii model iiic cuvettebased fluorometer with data max 2. For clarity, some data are represented as percentages, but all comparisons were made with raw data. Nitrite assay in conditioned media because no is labile, its production could not be measured directly but was assessed from. Absorbance at a wavelength of 450 nm were measured with a victor3 multilabel reader perkin elmer, waltham, ma. For fluorescence microscopic observation of intracellular ros production upon odorant stimulation in osn cultures, osns were loaded with 10. The wallac victor 2 is a high performance multilabel plate reader. Upstream open reading frames cause widespread reduction of.
Pdf small molecule inhibitors of apaf1related caspase 3. We use cookies to ensure that we give you the best experience on our website. Austin weigle graduate research assistant university of. The wallac 1420 victor2 is a multilabel, multitask plate reader designed to support the future demands of industrial and academic laboratories for multiple assay technologies on a single. High stability and fast recovery of expression of the tol. Differences between rlus were tested by the twotailed unpaired students ttest. The optical density values were converted to digital data with the wallac 1420 workstation software perkin elmer. Evaluation of cell toxicity and flow cytometry analysis. Absorbance, or overall plate functional activity, was measured at 590 nm every 12 h up to 120 h or until the average plate absorbance reached 0. The wallac 1420 victor2 is a multilabel, multitask plate reader designed to support the future demands of industrial and academic laboratories for multiple assay technologies on a single platform. Perkin elmer wallac 1420 victor2 microplate reader gmi trusted.
Victor x2 provides luminescence and fluorescence intensity fi detection technologies. The k d values were calculated using a two state model. Refurbished perkin elmerwallac victor 2 multilabel counter. All animal experiments were performed under protocols approved by the city of hope institutional animal care and use committee.
An extended version of the successful wallac victor multilabel reader, the victor2 allows immediate access to more than 10 counting modes. Wallac 1420 d workstation perkinelmer life sciences. Calcium flux was monitored continuously, both before and after addition of agonist or drug indicated by the arrow, for a total of 612 minutes, at 535 and 660 nm, using a fluorescence plate reader and wallac 1420 workstation software wallac victor 2 1420 multilabel counter, perkin elmer. P2000 serverworkstation software installation manual.
Small molecule inhibitors of apaf1related caspase 39 activation that control mitochondrialdependent apoptosis article pdf available in cell death and differentiation 9. For each well, the average cps of three consecutive measurements per well was used, using the software wallac 1420 workstation. Data were recorded by using a wallac 1420 workstation software. Operates as a standalone reader or with inputoutput. Ease of use and general reliability are enhanced by 32bit windows workstation software, designed to utilize the full. Wallac 1420 multilabel counter is a complete platform for. The data analysis was performed using wallac 1420 workstation software wallac 1420 manager, version 2. Apr 22, 2015 fluorescence measurements were taken using wallac 1420 workstation software version 2. The wallac 1420 instrument server can not be started. The wallac 1420 victor 2 is a multilabel, multitask plate reader designed to support the future demands of industrial and academic laboratories for multiple assay technologies on a single platform. Perkinelmer has led the way for over 25 years with powerful software and informatics solutions.
Bcl2 bcell lymphoma 2 proteins are key regulators of apoptosis. Development of a highthroughput method to evaluate serum. Used wallac victor 2 1420 microplate reader for sale dotmed. The samples were measured on a wallac victor 1420 multilabel counter using wallac 1420 workstation software version 2 wallac oy, turku, finland. Copy left click whichever protocol you would like to use. H 2 o 2 was used as a standard for intracellular ros production. Instrument manual perkinelmer 2030 multilabel reader 2030902001 march 2008 perkinelmer 2030 multilabel reader valid for instruments with software version 4.
We will be glad if you go back to us again and again. The victor 3v model 1420041 is the topoftheline model in the victor series. With powerful software and enhanced performance, victor x gives you the power of a bestinclass reader that works within your budget. Victor x light provides luminescence detection technology. An extended version of the successful wallac victor multilabel reader, the victor3 allows immediate access to more than 10 counting modes. A printed report is provided by each counter by means of printers controlled by the counters software. Users can take advantage of multiple assay technologies fitted in the wallac 1420 victor2 microplate reader. You can switch between normal and demo modes using the options selection in the tools menu. Twosided, homoscedastic t tests assessed significance. Fluorescence measurements were taken using wallac 1420 workstation software version 2. Following 2 h incubation at 37 c, with orbital shaking 450 rpm, the absorbance of each well was recorded at 600 nm using a multiplate reader perkin elmer, victor 3 v with wallac 1420 workstation software.